Abstract: Objective To create a mouse model of eosinophilic gastroenteritis induced by ovalbumin. Methods Twenty 6-week-old female BALB/c mice were randomly divided into experiment and control groups. The experiment group was intraperitoneally injected with ovalbumin (OVA) and Al(OH)3 as basic sensitization on day 1, and then intraperitoneally injected with OVA and normal saline as strengthening sensitization on day 15. The control group was intraperitoneally injected the same volume of normal saline. During days 18 to 30, the experiment group was challenged 7 times by intragastric injection of OVA encapsulated by PLGA and normal saline every other day . The control group was intragastrically injected with same volume of normal saline. During the molding period, fecal occult blood was detected and activity index score was counted. On day  31, all mice were sacrificed. The serum eosinophils (EOS) was detected. The tissues of stomach, ileum and colon were taken to evaluate the pathological changes and EOS density under light microscope. Results Mice in the experiment group showed piloerection, roachback and lazy. The rat’s weigh was not different between two groups. The score of DAI was higher in experiment group than that in control group (P < 0.01). The EOS count in peripheral blood was not different between two groups (P > 0.05). Under light microscope, the density of ESO in gastrointestinal mucosa was higher in experiment group than that in control group (P < 0.05). The injury was more severe in gastrointestinal mucosa in experiment group than that in control group. Conclusion Intraperitoneal injection of OVA and Al(OH)3 and then being challenged seven times by gavage with OVA wrapped by PLGA every other day, could successfully create a eosinophillic gastroenteritis model.